​​Effect of Different Media on in vitro Shoot Regeneration from Various Explants of Lilium longiflorum Cv. Elite, Brunello, Cordelia

Background: Lilium (Lilium longiflorum Thunb.) belongs to the family Liliaceae and is a native of Northern Hemisphere (up South Canada Siberia). Conventionally, lilium can easily be propagated by sexual asexual methods propagation but these prevalent are not capable meeting increasing demand in domestic global market. Generally, through bulbs which has bottlenecks like high cost planting material, infected from seed borne other diseases mosaic, grey mould, etc. So, vitro multiplication utmost important. Lily regenerated explants The objective present study was identify best media explant among scales nodal segments belonging three different cultivars viz., Elite, Brunello, Cordelia where maximum number shoots could regenerated. Methods: investigation carried out tissue Culture Laboratory Centre for Research Application Plant Tissue Culture. Twenty-five combinations were used their effect on shoot regeneration various cultivars. Two type viz. aseptically excised Lilum cultured Murashige Skoog (MS) medium containing concentrations 6-benzyl amino purine (BAP), kinetin Naphthalene Acetic Acid (NAA). Result: In Elite observed when combination i.e., LM-8 (MS+BAP 3.0 mg/l+NAA0.1 mg/l). cultivar Cordelia, showed response LM-24 (MS+Kinetin 2.0 mg/l+NAA 0.1